Nature Methods publishes our survey and analysis of peer-reviewed qPCR literature
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All you ever wanted to know about real-time quantitative PCR
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About the author
Stephen Bustin BA(Mod) PhD FSB obtained his PhD from Trinity College Dublin and has been working with PCR since 1987. In 2012 he was appointed Professor of Allied Health and Medicine at Anglia Ruskin University, having been Professor of Molecular Science at Queen Mary University of London, where he retains an honorary Chair. He also was visiting Professor of Molecular Biology at the University of Middlesex until 2013. He acquired his first qPCR instrument in 1997 and has published numerous peer-reviewed papers that describe and use this technology. He co-edited the “A-Z of Quantitative PCR” (2004), “The PCR Revolution” (2011) and "PCR Technology: Current Innovations 3rd Edition" (2013). He led the international consortium that drew up the MIQE guidelines (2009) and is in constant demand as a speaker and teacher at international qPCR meetings and workshops. At the 2007 Autism trial at the Office of Special Masters of the U.S. Court of Federal Claims he was an expert witness for the Department of Justice in the Cedillo v. Secretary of Health and Human Services case (#98-916V). He was described as "one of the most highly qualified and credible expert witnesses I [the Special Master] have ever encountered" (p.33).